fluorescent tubulin  (Cytoskeleton Inc)

Journal: eLife

Article Title: Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

doi: 10.7554/eLife.109308

Figure Lengend Snippet: Representative movie from MD simulations showing interactions of the Y-αCTT with the tubulin body over 240 ns. The tubulin body is shown in cartoon and colored gray. The αCTT is shown in stick and colored yellow with the glutamate side chains in red. Residues in site 1 (green), site 2 (cyan), and site 3 (magenta) appear as spheres when the αCTT is forming salt bridges with those residues.

Article Snippet: Peptide, recombinant protein , HiLyte488 porcine brain tubulin , Cytoskeleton , Cat# TL488M , .

Techniques:

Journal: Nature Communications

Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau

doi: 10.1038/s41467-025-67888-x

Figure Lengend Snippet: A Snapshots showing the dynamic gliding of microtubules ( cyan , labeled with HiLyte 488,) along DNA ( green , stained with SYTOX Orange), driven by the mobility of tau condensates ( magenta , labeled with Cy5) on DNA. Microtubules were formed by incubating with 5 μM tubulin. B Schematic illustration of the DNA–tau–microtubule tripartite assembly. C Representative kymograph displaying the mobility of the gliding microtubule on DNA shown in ( A ). D Time-lapse images capturing the pivoting motion of the microtubule, anchored by tau–DNA co-condensates. In ( A ) and ( B ), red boxes mark the location of the DNA strands, and white dashed lines show the position of the microtubules in the preceding image. E – G TIRF images of high-density surface-tethered λ-DNA ( green , stained with SYTOX Orange) before tau addition ( E ), after adding 5 μM tau ( magenta , Cy5-labeled) with 5% PEG ( F ), and after adding a mixture of tau and tubulin ( cyan , HiLyte 488-labeled) with 5% PEG ( G ). Microtubules were pulled to the surface by tau–DNA co-condensates. H , I Large-area views of microtubule pulldown on DNA surfaces with and without preformed tau–DNA co-condensates. In ( H ), microtubules did not attach to the DNA surface without tau pre-incubation to form condensates; only background fluorescence from out-of-focus tubulins was visible. J Quantification of surface-bound microtubules with and without preformed tau–DNA co-condensates ( n = 9 images; mean ± s.d.).

Article Snippet: After co-condensate formation, HiLyte 488-labeled tubulin (Cytoskeleton, #TL488M-A) was mixed with unlabeled tubulin (Cytoskeleton, #T240-B), making up 4% of the total tubulin concentration.

Techniques: Labeling, Staining, Incubation, Fluorescence